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Journal of Biomedical Optics • Open Access

Multifrequency synthesis and extraction using square wave projection patterns for quantitative tissue imaging

Paper Abstract

We present a method for spatial frequency domain data acquisition utilizing a multifrequency synthesis and extraction (MSE) method and binary square wave projection patterns. By illuminating a sample with square wave patterns, multiple spatial frequency components are simultaneously attenuated and can be extracted to determine optical property and depth information. Additionally, binary patterns are projected faster than sinusoids typically used in spatial frequency domain imaging (SFDI), allowing for short (millisecond or less) camera exposure times, and data acquisition speeds an order of magnitude or more greater than conventional SFDI. In cases where sensitivity to superficial layers or scattering is important, the fundamental component from higher frequency square wave patterns can be used. When probing deeper layers, the fundamental and harmonic components from lower frequency square wave patterns can be used. We compared optical property and depth penetration results extracted using square waves to those obtained using sinusoidal patterns on an <italic<in vivo</italic< human forearm and absorbing tube phantom, respectively. Absorption and reduced scattering coefficient values agree with conventional SFDI to within 1% using both high frequency (fundamental) and low frequency (fundamental and harmonic) spatial frequencies. Depth penetration reflectance values also agree to within 1% of conventional SFDI.

Paper Details

Date Published: 2 November 2015
PDF: 10 pages
J. Biomed. Opt. 20(11) 116005 doi: 10.1117/1.JBO.20.11.116005
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Kyle P. Nadeau, Beckman Laser Institute and Medical Clinic (United States)
Tyler B. Rice, Beckman Laser Institute (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)


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