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Journal of Biomedical Optics

Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses
Author(s): Aisada Uchugonova; Hans Georg Breunig; Ana Batista; Karsten König
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Paper Abstract

We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.

Paper Details

Date Published: 30 November 2015
PDF: 7 pages
J. Biomed. Opt. 20(11) 115008 doi: 10.1117/1.JBO.20.11.115008
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Aisada Uchugonova, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)
Hans Georg Breunig, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)
Ana Batista, Univ. des Saarlandes (Germany)
Karsten König, Univ. des Saarlandes (Germany)
JenLab GmbH (Germany)

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