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Journal of Biomedical Optics

Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation
Author(s): Stefan Kalies; Sebastian Keil; Sina Sender; Susanne C. Hammer; Georgios C. Antonopoulos; Markus Schomaker; Tammo Ripken; Hugo Murua Escobar; Heiko Meyer; Dag Heinemann
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Paper Abstract

Laser-based transfection techniques have proven high applicability in several cell biologic applications. The delivery of different molecules using these techniques has been extensively investigated. In particular, new high-throughput approaches such as gold nanoparticle–mediated laser transfection allow efficient delivery of antisense molecules or proteins into cells preserving high cell viabilities. However, the cellular response to the perforation procedure is not well understood. We herein analyzed the perforation kinetics of single cells during resonant gold nanoparticle–mediated laser manipulation with an 850-ps laser system at a wavelength of 532 nm. Inflow velocity of propidium iodide into manipulated cells reached a maximum within a few seconds. Experiments based on the inflow of FM4-64 indicated that the membrane remains permeable for a few minutes for small molecules. To further characterize the cellular response postmanipulation, we analyzed levels of oxidative heat or general stress. Although we observed an increased formation of reactive oxygen species by an increase of dichlorofluorescein fluorescence, heat shock protein 70 was not upregulated in laser-treated cells. Additionally, no evidence of stress granule formation was visible by immunofluorescence staining. The data provided in this study help to identify the cellular reactions to gold nanoparticle–mediated laser manipulation.

Paper Details

Date Published: 12 November 2015
PDF: 8 pages
J. Biomed. Opt. 20(11) 115005 doi: 10.1117/1.JBO.20.11.115005
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Stefan Kalies, Laser Zentrum Hannover e.V. (Germany)
Sebastian Keil, Laser Zentrum Hannover e.V. (Germany)
Sina Sender, Laser Zentrum Hannover e.V. (Germany)
Susanne C. Hammer, StiftungTierärztliche Hochschule Hannover (Germany)
Univ. of Rostock (Germany)
Georgios C. Antonopoulos, Laser Zentrum Hannover e.V. (Germany)
Markus Schomaker, Laser Zentrum Hannover e.V. (Germany)
Tammo Ripken, Laser Zentrum Hannover e.V. (Germany)
Hugo Murua Escobar, StiftungTierärztliche Hochschule Hannover (Germany)
Univ. of Rostock (Germany)
Heiko Meyer, Laser Zentrum Hannover e.V. (Germany)
Dag Heinemann, Laser Zentrum Hannover e.V. (Germany)


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