Share Email Print
cover

Journal of Biomedical Optics

Differences in forward angular light scattering distributions between M1 and M2 macrophages
Author(s): David L. Halaney; Aydin Zahedivash; Jennifer E. Phipps; Tianyi Wang; Jordan Dwelle; Claude Jourdan Le Saux; Reto Asmis; Thomas E. Milner; Marc D. Feldman
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

The ability to distinguish macrophage subtypes noninvasively could have diagnostic potential in cancer, atherosclerosis, and diabetes, where polarized M1 and M2 macrophages play critical and often opposing roles. Current methods to distinguish macrophage subtypes rely on tissue biopsy. Optical imaging techniques based on light scattering are of interest as they can be translated into biopsy-free strategies. Because mitochondria are relatively strong subcellular light scattering centers, and M2 macrophages are known to have enhanced mitochondrial biogenesis compared to M1, we hypothesized that M1 and M2 macrophages may have different angular light scattering profiles. To test this, we developed an <italic<in vitro</italic< angle-resolved forward light scattering measurement system. We found that M1 and M2 macrophage monolayers scatter relatively unequal amounts of light in the forward direction between 1.6 deg and 3.2 deg with M2 forward scattering significantly more light than M1 at increasing angles. The ratio of forward scattering can be used to identify the polarization state of macrophage populations in culture.

Paper Details

Date Published: 5 November 2015
PDF: 9 pages
J. Biomed. Opt. 20(11) 115002 doi: 10.1117/1.JBO.20.11.115002
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
David L. Halaney, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
South Texas Veterans Health Care System (United States)
Aydin Zahedivash, The Univ. of Texas at Austin (United States)
Jennifer E. Phipps, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Tianyi Wang, The Univ. of Texas at Austin (United States)
Jordan Dwelle, JP3 Measurement (United States)
South Texas Veterans Health Care System (United States)
The Univ. of Texas at Austin (United States)
Claude Jourdan Le Saux, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Reto Asmis, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Thomas E. Milner, The Univ. of Texas at Austin (United States)
Marc D. Feldman, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
South Texas Veterans Health Care System (United States)


© SPIE. Terms of Use
Back to Top