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Journal of Biomedical Optics

Quantitative investigation of red blood cell three-dimensional geometric and chemical changes in the storage lesion using digital holographic microscopy
Author(s): Keyvan Jaferzadeh; Inkyu Moon
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Paper Abstract

Quantitative phase information obtained by digital holographic microscopy (DHM) can provide new insight into the functions and morphology of single red blood cells (RBCs). Since the functionality of a RBC is related to its three-dimensional (3-D) shape, quantitative 3-D geometric changes induced by storage time can help hematologists realize its optimal functionality period. We quantitatively investigate RBC 3-D geometric changes in the storage lesion using DHM. Our experimental results show that the substantial geometric transformation of the biconcave-shaped RBCs to the spherocyte occurs due to RBC storage lesion. This transformation leads to progressive loss of cell surface area, surface-to-volume ratio, and functionality of RBCs. Furthermore, our quantitative analysis shows that there are significant correlations between chemical and morphological properties of RBCs.

Paper Details

Date Published: 21 October 2015
PDF: 10 pages
J. Biomed. Opt. 20(11) 111218 doi: 10.1117/1.JBO.20.11.111218
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Keyvan Jaferzadeh, Chosun Univ. (Republic of Korea)
Inkyu Moon, Chosun Univ. (Republic of Korea)

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