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Journal of Biomedical Optics

Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing
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Paper Abstract

We propose a new portable imaging configuration that can double the field of view (FOV) of existing off-axis interferometric imaging setups, including broadband off-axis interferometers. This configuration is attached at the output port of the off-axis interferometer and optically creates a multiplexed interferogram on the digital camera, which is composed of two off-axis interferograms with straight fringes at orthogonal directions. Each of these interferograms contains a different FOV of the imaged sample. Due to the separation of these two FOVs in the spatial-frequency domain, they can be fully reconstructed separately, while obtaining two complex wavefronts from the sample at once. Since the optically multiplexed off-axis interferogram is recorded by the camera in a single exposure, fast dynamics can be recorded with a doubled imaging area. We used this technique for quantitative phase microscopy of biological samples with extended FOV. We demonstrate attaching the proposed module to a diffractive phase microscopy interferometer, illuminated by a broadband light source. The biological samples used for the experimental demonstrations include microscopic diatom shells, cancer cells, and flowing blood cells.

Paper Details

Date Published: 6 October 2015
PDF: 6 pages
J. Biomed. Opt. 20(11) 111217 doi: 10.1117/1.JBO.20.11.111217
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Pinhas Girshovitz, Tel Aviv Univ. (Israel)
Irena Frenklach, Tel Aviv Univ. (Israel)
Natan T. Shaked, Tel Aviv Univ. (Israel)

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