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Journal of Biomedical Optics

Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior
Author(s): Aneta Křížová; Jana Čolláková; Zbynek Dostal; Lukas Kvasnica; Hana Uhlirova; Tomáš Zikmund; Pavel Veselý; Radim Chmelik
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Paper Abstract

Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two-dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed.

Paper Details

Date Published: 3 September 2015
PDF: 8 pages
J. Biomed. Opt. 20(11) 111214 doi: 10.1117/1.JBO.20.11.111214
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Aneta Křížová, CEITEC Brno Univ. of Technology (Czech Republic)
Jana Čolláková, CEITEC Brno Univ. of Technology (Czech Republic)
Zbynek Dostal, CEITEC Brno Univ. of Technology (Czech Republic)
Lukas Kvasnica, Brno Univ. of Technology (Czech Republic)
Hana Uhlirova, Univ. of California, San Diego (United States)
Tomáš Zikmund, CEITEC Brno Univ. of Technology (Czech Republic)
Pavel Veselý, CEITEC Brno Univ. of Technology (Czech Republic)
Radim Chmelik, CEITEC Brno Univ. of Technology (Czech Republic)


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