Share Email Print

Journal of Biomedical Optics

Quantitative phase imaging of arthropods
Author(s): Shamira Sridharan; Aron Katz; Felipe Soto-Adames; Gabriel Popescu
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Classification of arthropods is performed by characterization of fine features such as setae and cuticles. An unstained whole arthropod specimen mounted on a slide can be preserved for many decades, but is difficult to study since current methods require sample manipulation or tedious image processing. Spatial light interference microscopy (SLIM) is a quantitative phase imaging (QPI) technique that is an add-on module to a commercial phase contrast microscope. We use SLIM to image a whole organism springtail Ceratophysella denticulata mounted on a slide. This is the first time, to our knowledge, that an entire organism has been imaged using QPI. We also demonstrate the ability of SLIM to image fine structures in addition to providing quantitative data that cannot be obtained by traditional bright field microscopy.

Paper Details

Date Published: 3 September 2015
PDF: 6 pages
J. Biomed. Opt. 20(11) 111212 doi: 10.1117/1.JBO.20.11.111212
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Shamira Sridharan, Univ. of Illinois at Urbana-Champaign (United States)
Aron Katz, Univ. of Illinois at Urbana-Champaign (United States)
Felipe Soto-Adames, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)

© SPIE. Terms of Use
Back to Top