Share Email Print

Journal of Biomedical Optics • Open Access

Simultaneous full-field 3-D vibrometry of the human eardrum using spatial-bandwidth multiplexed holography
Author(s): Morteza Khaleghi; Jérémie Guignard; Cosme Furlong; John J. Rosowski

Paper Abstract

Holographic interferometric methods typically require the use of three sensitivity vectors in order to obtain three-dimensional (3-D) information. Methods based on multiple directions of illumination have limited applications when studying biological tissues that have temporally varying responses such as the tympanic membrane (TM). Therefore, to measure 3-D displacements in such applications, the measurements along all the sensitivity vectors have to be done simultaneously. We propose a multiple-illumination directions approach to measure 3-D displacements from a single-shot hologram that contains displacement information from three sensitivity vectors. The hologram of an object of interest is simultaneously recorded with three incoherently superimposed pairs of reference and object beams. The incident off-axis angles of the reference beams are adjusted such that the frequency components of the multiplexed hologram are completely separate. Because of the differences in the directions and wavelengths of the reference beams, the positions of each reconstructed image corresponding to each sensitivity vector are different. We implemented a registration algorithm to accurately translate individual components of the hologram into a single global coordinate system to calculate 3-D displacements. The results include magnitudes and phases of 3-D sound-induced motions of a human cadaveric TM at several excitation frequencies showing modal and traveling wave motions on its surface.

Paper Details

Date Published: 18 May 2015
PDF: 11 pages
J. Biomed. Opt. 20(11) 111202 doi: 10.1117/1.JBO.20.11.111202
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Morteza Khaleghi, Worcester Polytechnic Institute (United States)
Jérémie Guignard, Massachusetts Eye and Ear Infirmary (United States)
Harvard Medical School (United States)
Cosme Furlong, Worcester Polytechnic Institute (United States)
Massachusetts Eye and Ear Infirmary (United States)
Harvard Medical School (United States)
John J. Rosowski, Massachusetts General Hospital (United States)
Harvard Medical School (United States)

© SPIE. Terms of Use
Back to Top