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Journal of Biomedical Optics

Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology
Author(s): Qingli Li; Hui Peng; Jing Wang; Yiting Wang; Fangmin Guo
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Paper Abstract

A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes.

Paper Details

Date Published: 20 November 2015
PDF: 4 pages
J. Biomed. Opt. 20(11) 110504 doi: 10.1117/1.JBO.20.11.110504
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Qingli Li, East China Normal Univ. (China)
Hui Peng, East China Normal Univ. (China)
Jing Wang, East China Normal Univ. (China)
Yiting Wang, East China Normal Univ. (China)
Fangmin Guo, East China Normal Univ. (China)

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