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Journal of Biomedical Optics

Integrated femtosecond stimulated Raman scattering and two-photon fluorescence imaging of subcellular lipid and vesicular structures
Author(s): Xuesong Li; Wen Jiun Lam; Zhe Cao; Yan Hao; Qiqi Sun; Sicong He; Ho Yi Mak; Jianan Y. Qu
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Paper Abstract

The primary goal of this study is to demonstrate that stimulated Raman scattering (SRS) as a new imaging modality can be integrated into a femtosecond (fs) nonlinear optical (NLO) microscope system. The fs sources of high pulse peak power are routinely used in multimodal nonlinear microscopy to enable efficient excitation of multiple NLO signals. However, with fs excitations, the SRS imaging of subcellular lipid and vesicular structures encounters significant interference from proteins due to poor spectral resolution and a lack of chemical specificity, respectively. We developed a unique NLO microscope of fs excitation that enables rapid acquisition of SRS and multiple two-photon excited fluorescence (TPEF) signals. In the

Paper Details

Date Published: 18 November 2015
PDF: 4 pages
J. Biomed. Opt. 20(11) 110501 doi: 10.1117/1.JBO.20.11.110501
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Xuesong Li, Hong Kong Univ. of Science and Technology (China)
Wen Jiun Lam, Hong Kong Univ. of Science and Technology (China)
Zhe Cao, Hong Kong Univ. of Science and Technology (China)
Yan Hao, Hong Kong Univ. of Science and Technology (China)
Qiqi Sun, Hong Kong Univ. of Science and Technology (China)
Sicong He, Hong Kong Univ. of Science and Technology (China)
Ho Yi Mak, Hong Kong Univ. of Science and Technology (China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (China)


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