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Journal of Biomedical Optics

Effect of low-level laser therapy on irradiated parotid glands—study in mice
Author(s): Monique Dossena Acauan; Ana Paula Gomes; Aroldo Braga-Filho; Maria Antonia Zancanaro de Figueiredo; Karen Cherubini; Fernanda G. Salum
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Paper Abstract

The objective of this study was to evaluate the effect of low-level laser therapy (LLLT) on radiotherapy-induced morphological changes and caspase-3 immunodetection in parotids of mice. Forty-one Swiss mice were divided into control, radiotherapy, 2- and 4-J laser groups. The experimental groups were exposed to ionizing radiation in a single session of 10 Gy. In the laser groups, a GaAlAs laser (830 nm, 100 mW, 0.028  cm2, 3.57  W/cm2) was used on the region corresponding to the parotid glands, with 2-J energy (20 s, 71  J/cm2) or 4 J (40 s, 135  J/cm2) per point. LLLT was performed immediately before and 24 h after radiotherapy. One point was applied in each parotid gland. The animals were euthanized 48 h or 7 days after radiotherapy and parotid glands were dissected for morphological analysis and immunodetection of caspase-3. There was no significant difference between groups in the immunodetection of caspase-3, but the laser groups had a lower percentage compared to the radiotherapy group. LLLT promoted the preservation of acinar structure, reduced the occurrence of vacuolation, and stimulated parotid gland vascularization. Of the two LLLT protocols, the one using 4 J of energy showed better results.

Paper Details

Date Published: 22 October 2015
PDF: 7 pages
J. Biomed. Opt. 20(10) 108002 doi: 10.1117/1.JBO.20.10.108002
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Monique Dossena Acauan, Pontifical Catholic Univ. of Rio de Janeiro (Brazil)
Ana Paula Gomes, UFPEL (Brazil)
Aroldo Braga-Filho, Pontifícia Univ. Católica do Rio Grande do Sul (Brazil)
Maria Antonia Zancanaro de Figueiredo, Pontifícia Univ. Católica do Rio Grande do Sul (Brazil)
Karen Cherubini, Pontifícia Univ. Católica do Rio Grande do Sul (Brazil)
Fernanda G. Salum, Pontifícia Univ. Católica do Rio Grande do Sul (Brazil)


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