Share Email Print

Journal of Biomedical Optics

Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats
Author(s): Hao Li; Wenzhong Liu; Hao F. Zhang
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Rodent models are indispensable in studying various retinal diseases. Noninvasive, high-resolution retinal imaging of rodent models is highly desired for longitudinally investigating the pathogenesis and therapeutic strategies. However, due to severe aberrations, the retinal image quality in rodents can be much worse than that in humans. We numerically and experimentally investigated the influence of chromatic aberration and optical illumination bandwidth on retinal imaging. We confirmed that the rat retinal image quality decreased with increasing illumination bandwidth. We achieved the retinal image resolution of 10  μm using a 19 nm illumination bandwidth centered at 580 nm in a home-built fundus camera. Furthermore, we observed higher chromatic aberration in albino rat eyes than in pigmented rat eyes. This study provides a design guide for high-resolution fundus camera for rodents. Our method is also beneficial to dispersion compensation in multiwavelength retinal imaging applications.

Paper Details

Date Published: 23 October 2015
PDF: 6 pages
J. Biomed. Opt. 20(10) 106010 doi: 10.1117/1.JBO.20.10.106010
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Hao Li, Northwestern Univ. (United States)
Wenzhong Liu, Northwestern Univ. (United States)
Hao F. Zhang, Northwestern Univ. (United States)

© SPIE. Terms of Use
Back to Top