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Journal of Biomedical Optics • Open Access

Noncontact photoacoustic tomography of <italic<in vivo</italic< chicken chorioallantoic membrane based on all-fiber heterodyne interferometry

Paper Abstract

We present three-dimensional (3-D) <italic<in vivo</italic< photoacoustic (PA) images of the blood vasculature of a chicken chorioallantoic membrane (CAM) obtained by using a fiber-based noncontact PA tomography system. With a fiber-optic heterodyne interferometer, the system measures the surface displacement of a sample, induced by the PA wave, which overcomes the disadvantage of physical-contact of ultrasonic transducer in a conventional system. The performance of an implemented system is analyzed and its capability of <italic<in vivo</italic< 3-D bioimaging is presented. At a depth of 2.5 mm in a phantom experiment, the lateral and axial resolutions were measured as 100 and <inline-formula< <mml:math display="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML"< <mml:mrow< <mml:mn<30</mml:mn< <mml:mtext< </mml:mtext< <mml:mtext< </mml:mtext< <mml:mi<μ</mml:mi< <mml:mi mathvariant="normal"<m</mml:mi< </mml:mrow< </mml:math< </inline-formula<, respectively. The lateral resolution became doubled at a depth of 7.0 mm; however, interestingly, the axial resolution was not noticeably deteriorated with the depth. With the CAM experiment, performed under the American National Standards Institute laser safety standard condition, blood vessel structures placed as deep as 3.5 mm were clearly recognized.

Paper Details

Date Published: 16 October 2015
PDF: 8 pages
J. Biomed. Opt. 20(10) 106007 doi: 10.1117/1.JBO.20.10.106007
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Jonghyun Eom, Gwangju Institute of Science and Technology (Korea, Republic of)
Seong Jun Park, Institute for Basic Science (Korea, Republic of)
Byeong Ha Lee, Gwangju Institute of Science and Technology (Korea, Republic of)


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