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Journal of Biomedical Optics

Laser line illumination scheme allowing the reduction of background signal and the correction of absorption heterogeneities effects for fluorescence reflectance imaging
Author(s): Frederic Fantoni; Lionel Hervé; Vincent Poher; Sylvain Gioux; Jérôme Mars; Jean-Marc Dinten
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Paper Abstract

Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality as it allows to noninvasively monitor the fluorescence targeted tumors located below the tissue surface. Some drawbacks of this technique are the background fluorescence decreasing the contrast and absorption heterogeneities leading to misinterpretations concerning fluorescence concentrations. We propose a correction technique based on a laser line scanning illumination scheme. We scan the medium with the laser line and acquire, at each position of the line, both fluorescence and excitation images. We then use the finding that there is a relationship between the excitation intensity profile and the background fluorescence one to predict the amount of signal to subtract from the fluorescence images to get a better contrast. As the light absorption information is contained both in fluorescence and excitation images, this method also permits us to correct the effects of absorption heterogeneities. This technique has been validated on simulations and experimentally. Fluorescent inclusions are observed in several configurations at depths ranging from 1 mm to 1 cm. Results obtained with this technique are compared with those obtained with a classical wide-field detection scheme for contrast enhancement and with the fluorescence by an excitation ratio approach for absorption correction.

Paper Details

Date Published: 7 October 2015
PDF: 11 pages
J. Biomed. Opt. 20(10) 106003 doi: 10.1117/1.JBO.20.10.106003
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Frederic Fantoni, Univ. Grenoble Alpes (France)
Lionel Hervé, Univ. Grenoble Alpes (France)
Vincent Poher, Univ. Grenoble Alpes (France)
Sylvain Gioux, Beth Israel Deaconess Medical Ctr. (United States)
Jérôme Mars, Institut National Polytechnique de Grenoble (France)
Jean-Marc Dinten, Univ. Grenoble Alpes (France)

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