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Journal of Biomedical Optics

Augmented microscopy: real-time overlay of bright-field and near-infrared fluorescence images
Author(s): Jeffrey R. Watson; Christian F. Gainer; Nikolay Martirosyan; Jesse Skoch; G. Michael Lemole; Rein Anton; Marek Romanowski
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Paper Abstract

Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed NIR fluorescence (synthetic) images are merged within the optical path of a stereomicroscope. Under luminance of 100,000 lx, representing typical illumination of the surgical field, the augmented microscope detects 189 nM concentration of indocyanine green and produces a composite of the real and synthetic images within the eyepiece of the microscope at 20 fps. Augmentation described here can be implemented as an add-on module to visualize NIR contrast agents, laser beams, or various types of electronic data within the surgical microscopes commonly used in neurosurgical, cerebrovascular, otolaryngological, and ophthalmic procedures.

Paper Details

Date Published: 6 October 2015
PDF: 9 pages
J. Biomed. Opt. 20(10) 106002 doi: 10.1117/1.JBO.20.10.106002
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Jeffrey R. Watson, The Univ. of Arizona (United States)
Christian F. Gainer, The Univ. of Arizona (United States)
Nikolay Martirosyan, The Univ. of Arizona (United States)
Jesse Skoch, The Univ. of Arizona (United States)
G. Michael Lemole, The Univ. of Arizona (United States)
Rein Anton, The Univ. of Arizona (United States)
Marek Romanowski, The Univ. of Arizona (United States)

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