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Journal of Biomedical Optics • Open Access

Mechanism of two-photon excited hemoglobin fluorescence emission
Author(s): Qiqi Sun; Wei Zheng; Jiannong Wang; Yi Luo; Jianan Y. Qu

Paper Abstract

Hemoglobin, one of the most important proteins in the human body, is composed of “heme” groups (iron-containing rings) and “globins” (proteins). We investigate the two-photon excited fluorescence of hemoglobin and its subunit components (heme and globin). We measure the hemoglobin fluorescence lifetime by using a streak camera of ps resolution and confirm that its lifetime is in femtosecond scale. In the study of the fluorescence properties of heme and globin, the experimental results reveal that heme is the sole fluorophore of hemoglobin. Hemoglobin fluorescence can be effectively excited only via two-photon process, because heme has a centrosymmetric molecular structure and two-photon allowed transition is forbidden for single-photon process and vice versa due to the Laporte parity selection rule.

Paper Details

Date Published: 27 October 2015
PDF: 6 pages
J. Biomed. Opt. 20(10) 105014 doi: 10.1117/1.JBO.20.10.105014
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Qiqi Sun, Hong Kong Univ. of Science and Technology (China)
Wei Zheng, Shenzhen Institute of Advanced Technology (China)
Jiannong Wang, Hong Kong Univ. of Science and Technology (China)
Yi Luo, Univ. of Science and Technology of China (China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (China)

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