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Journal of Biomedical Optics

Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans
Author(s): Tomoki Nishiguchi; Toshimichi Yamada; Yusuke Nasu; Mashiho Ito; Hideaki Yoshimura; Takeaki Ozawa
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Paper Abstract

Luciferase, a bioluminescent protein, has been used as an analytical tool to visualize intracellular phenomena. Luciferase with red light emission is particularly useful for bioluminescence imaging because of its high transmittance in mammalian tissues. However, the luminescence intensity of existing luciferases with their emission over 600 nm is insufficient for imaging studies because of their weak intensities. We developed mutants of Emerald luciferase (Eluc) from Brazilian click beetle (Pyrearinus termitilluminans), which emits the strongest bioluminescence among beetle luciferases. We successively introduced four amino acid mutations into the luciferase based on a predicted structure of Eluc using homology modeling. Results showed that quadruple mutations R214K/H241K/S246H/H347A into the beetle luciferase emit luminescence with emission maximum at 626 nm, 88-nm red-shift from the wild-type luciferase. This mutant luciferase is anticipated for application in in vivo multicolor imaging in living samples.

Paper Details

Date Published: 27 August 2015
PDF: 7 pages
J. Biomed. Opt. 20(10) 101205 doi: 10.1117/1.JBO.20.10.101205
Published in: Journal of Biomedical Optics Volume 20, Issue 10
Show Author Affiliations
Tomoki Nishiguchi, The Univ. of Tokyo (Japan)
Toshimichi Yamada, The Univ. of Tokyo (Japan)
Yusuke Nasu, The Univ. of Tokyo (Japan)
Mashiho Ito, The University of Tokyo (Japan)
Hideaki Yoshimura, The Univ. of Tokyo (Japan)
Takeaki Ozawa, The Univ. of Tokyo (Japan)

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