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Journal of Biomedical Optics • Open Access

Correlation of NADH fluorescence lifetime and oxidative phosphorylation metabolism in the osteogenic differentiation of human mesenchymal stem cell
Author(s): Han-Wen Guo; Jia-Sin Yu; Shu-Han Hsu; Yau-Huei Wei; Oscar K. Lee; Chen-Yuan Dong; Hsing-Wen Wang

Paper Abstract

Reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime has been broadly used as a metabolic indicator for stem cell imaging. However, the direct relationship between NADH fluorescence lifetime and metabolic pathway and activity remains to be clarified. In this study, we measured the NADH fluorescence lifetime of human mesenchymal stem cells (hMSCs) as well as the metabolic indictors, such as adenosine triphosphate (ATP) level, oxygen consumption, and lactate release, up to 4 weeks under normal osteogenic differentiation and oxidative phosphorylation-attenuated/inhibited differentiation by oligomycin A (OA) treatment. NADH fluorescence lifetime was positively correlated with oxygen consumption and ATP level during energy transformation from glycolysis to oxidative phosphorylation. Under OA treatment, oxidative phosphorylation was attenuated/inhibited (i.e., oxygen consumption remained the same as controls or lower), cells showed attenuated differentiation under glycolysis, and NADH fluorescence lifetime change was not detected. Increased expression of the overall complex proteins was observed in addition to Complex I. We suggested special caution needs to be exercised while interpreting NADH fluorescence lifetime signal in terms of stem cell differentiation.

Paper Details

Date Published: 28 January 2015
PDF: 8 pages
J. Biomed. Opt. 20(1) 017004 doi: 10.1117/1.JBO.20.1.017004
Published in: Journal of Biomedical Optics Volume 20, Issue 1
Show Author Affiliations
Han-Wen Guo, National Yang-Ming Univ. (Taiwan)
National Taiwan Univ. (Taiwan)
Jia-Sin Yu, National Yang-Ming Univ. (Taiwan)
Shu-Han Hsu, National Yang-Ming Univ. (Taiwan)
Yau-Huei Wei, National Yang-Ming Univ. (Taiwan)
Mackay Medical College (Taiwan)
Oscar K. Lee, National Yang-Ming Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Hsing-Wen Wang, National Yang-Ming Univ. (Taiwan)
Univ. of Maryland (United States)

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