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Journal of Biomedical Optics • Open Access

Hydrophobic cyanine dye-doped micelles for optical in vivo imaging of plasma leakage and vascular disruption
Author(s): Bálint Botz; Kata Bölcskei; Ágnes Kemény; Zoltán Sándor; Valéria Tékus; György Sétáló; Janka Csepregi; Attila Mócsai; Erika Pintér; László Kollár; Zsuzsanna Helyes

Paper Abstract

Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats. The imaging formula is stable and exerts no acute toxic effects in vitro. It accumulated specifically in the inflamed regions in all models, which could be demonstrated by both conventional epifluorescence imaging, and fluorescence tomography both as a standalone technique and also by merging it with computed tomography scans. Ex vivo verification of dye accumulation by confocal fluorescence microscopy was also possible. The present formula allows sensitive and specific detection of inflammatory plasma leakage in diverse models. Its potential for imaging larger animals was also demonstrated. IR-676-doped micelles offer an excellent opportunity to image inflammatory vascular leakage in various models and species.

Paper Details

Date Published: 28 January 2015
PDF: 9 pages
J. Biomed. Opt. 20(1) 016022 doi: 10.1117/1.JBO.20.1.016022
Published in: Journal of Biomedical Optics Volume 20, Issue 1
Show Author Affiliations
Bálint Botz, Univ. of Pécs (Hungary)
Kata Bölcskei, Univ. of Pécs (Hungary)
Ágnes Kemény, Univ. of Pécs (Hungary)
Zoltán Sándor, Univ. of Pécs (Hungary)
Valéria Tékus, Univ. of Pécs (Hungary)
György Sétáló, Univ. of Pécs (Hungary)
Janka Csepregi, Semmelweis Univ. (Hungary)
Attila Mócsai, Semmelweis Univ. (Hungary)
Erika Pintér, Univ. of Pécs (Hungary)
László Kollár, Univ. of Pécs (Hungary)
Zsuzsanna Helyes, Univ. of Pécs (Hungary)

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