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Journal of Biomedical Optics

Visualizing and quantifying difference in cytoplasmic and nuclear metabolism in the hepatobiliary system in vivo
Author(s): Chih-Ju Lin; Ning Kang; Jian-Ye Lee; Hsuan-Shu Lee; Chen-Yuan Dong
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Paper Abstract

The liver is a major organ responsible for performing xenobiotic metabolism. In this process, xenobiotic is uptaken and processed in hepatocytes and subsequently excreted into the bile canaliculi. However, the intracellular heterogeneity in such metabolic processes is not known. We use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to investigate xenobiotic metabolism in hepatocytes with intravital multiphoton fluorescence microscopy. 6-CFDA is processed by intracellular esterase to fluorescent 6-CF, which can be imaged and quantified. We found that compared to the nucleus, cytoplasmic 6-CF fluorescence intensity reached a maximum earlier (cytoplasm: 11.3±4.4  min; nucleus: 14.7±4.9  min) following 6-CFDA injection. We also found a slight difference in the rate of 6-CFDA metabolism as the rates of 6-CF decay at rates of 1.43±0.75 and 1.27±0.72  photons/min for the cytoplasm and nucleus, respectively. These results indicate that molecular transport to the nucleus is additionally hindered and can affect drug transport there.

Paper Details

Date Published: 22 January 2015
PDF: 4 pages
J. Biomed. Opt. 20(1) 016020 doi: 10.1117/1.JBO.20.1.016020
Published in: Journal of Biomedical Optics Volume 20, Issue 1
Show Author Affiliations
Chih-Ju Lin, National Taiwan Univ. (Taiwan)
Ning Kang, National Taiwan Univ. (Taiwan)
Jian-Ye Lee, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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