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Journal of Biomedical Optics • Open Access

Fluorescence imaging of dendritic spines of Golgi-Cox-stained neurons using brightening background
Author(s): Min Ai; Hanqing Xiong; Tao Yang; Zhenhua Shang; Muqing Chen; Xiuli Liu; Shaoqun Zeng

Paper Abstract

We report a novel fluorescence imaging approach to imaging nonfluorescence-labeled biological tissue samples. The method was demonstrated by imaging neurons in Golgi-Cox-stained and epoxy-resin-embedded samples through the excitation of the background fluorescence of the specimens. The dark neurons stood out clearly against background fluorescence in the images, enabling the tracing of a single dendritic spine using both confocal and wide-field fluorescence microscopy. The results suggest that the reported fluorescence imaging method would provide an effective alternative solution to image nonfluorescence-labeled samples, and it allows tracing the dendritic spine structure of neurons.

Paper Details

Date Published: 13 January 2015
PDF: 3 pages
J. Biomed. Opt. 20(1) 010501 doi: 10.1117/1.JBO.20.1.010501
Published in: Journal of Biomedical Optics Volume 20, Issue 1
Show Author Affiliations
Min Ai, Britton Chance Ctr. for Biomedical Photonics (China)
Hanqing Xiong, Britton Chance Ctr. for Biomedical Photonics (China)
Tao Yang, Britton Chance Ctr. for Biomedical Photonics (China)
Zhenhua Shang, Britton Chance Ctr. for Biomedical Photonics (China)
Muqing Chen, Hubei Univ. of Education (China)
Xiuli Liu, Britton Chance Ctr. for Biomedical Photonics (China)
Shaoqun Zeng, Britton Chance Ctr. for Biomedical Photonics (China)


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