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Journal of Biomedical Optics

Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters
Author(s): Matias E. Mantineo; João Páscoa Pinheiro; António Miguel Morgado
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Paper Abstract

We evaluated the effect of different irradiation parameters in low-level laser therapy (LLLT) for treating inflammation induced in the gastrocnemius muscle of rats through cytokines concentration in systemic blood and analysis of muscle tissue. We used continuous (830 and 980 nm) and pulsed illuminations (830 nm). Animals were divided into five groups per wavelength (10, 20, 30, 40, and 50 mW), and a control group. LLLT was applied during 5 days with a constant irradiation time and area. TNF-α , IL-1β , IL-2, and IL-6 cytokines were quantified by ELISA. Inflammatory cells were counted using microscopy. Identical methodology was used with pulsed illumination. Average power (40 mW) and duty cycle were kept constant (80%) at five frequencies (5, 25, 50, 100, and 200 Hz). For continuous irradiation, treatment effects occurred for all doses, with a reduction of TNF-α , IL-1β , and IL-6 cytokines and inflammatory cells. Continuous irradiation at 830 nm was more effective, a result explained by the action spectrum of cytochrome c oxidase (CCO). Best results were obtained for 40 mW, with data suggesting a biphasic dose response. Pulsed wave irradiation was only effective for higher frequencies, a result that might be related to the rate constants of the CCO internal electron transfer process.

Paper Details

Date Published: 8 September 2014
PDF: 12 pages
J. Biomed. Opt. 19(9) 098002 doi: 10.1117/1.JBO.19.9.098002
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Matias E. Mantineo, Univ. de Coimbra (Portugal)
Institute for Biomedical Imaging and Life Sciences (Portugal)
João Páscoa Pinheiro, Univ. de Coimbra (Portugal)
António Miguel Morgado, Univ. de Coimbra (Portugal)
Institute for Biomedical Imaging and Life Sciences (Portugal)

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