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Journal of Biomedical Optics

Photonic detection and characterization of DNA using sapphire microspheres
Author(s): Mohammed Sharif Murib; Weng-Siang Yeap; Daan Martens; Peter Bienstman; Ward De Ceuninck; Bart van Grinsven; Michael J. Schöning; Luc Michiels; Ken Haenen; Marcel Ameloot; Ali Serpengüzel; Patrick Wagner
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Paper Abstract

A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500  μm, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q≈104) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants.

Paper Details

Date Published: 26 September 2014
PDF: 7 pages
J. Biomed. Opt. 19(9) 097006 doi: 10.1117/1.JBO.19.9.097006
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Mohammed Sharif Murib, Univ. Hasselt (Belgium)
Weng-Siang Yeap, Univ. Hasselt (Belgium)
Daan Martens, Univ. Gent (Belgium)
Peter Bienstman, Univ. Gent (Belgium)
Ward De Ceuninck, Hasselt Univ. (Belgium)
Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk (Belgium)
Bart van Grinsven, Univ. Hasselt (Belgium)
Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk (Belgium)
Michael J. Schöning, RWTH Aachen Univ. (Germany)
Luc Michiels, Univ. Hasselt (Belgium)
Ken Haenen, Univ. Hasselt (Belgium)
Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk (Belgium)
Marcel Ameloot, Univ. Hasselt (Belgium)
Ali Serpengüzel, Koç Univ. (Turkey)
Patrick Wagner, Univ. Hasselt (Belgium)
Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk (Belgium)

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