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Journal of Biomedical Optics

Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing
Author(s): Tae-Youl Choi; Michael L. Denton; Gary D. Noojin; Larry E. Estlack; Ramesh L. Shrestha; Benjamin A. Rockwell; Robert J. Thomas; Dongsik Kim
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Paper Abstract

A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4  μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73°C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.

Paper Details

Date Published: 15 September 2014
PDF: 4 pages
J. Biomed. Opt. 19(9) 097003 doi: 10.1117/1.JBO.19.9.097003
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Tae-Youl Choi, Univ. of North Texas (United States)
Michael L. Denton, TASC, Inc. (United States)
Gary D. Noojin, TASC, Inc. (United States)
Larry E. Estlack, Conceptual MindWorks, Inc. (United States)
Ramesh L. Shrestha, Univ. of North Texas (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)
Robert J. Thomas, Air Force Research Lab. (United States)
Dongsik Kim, Pohang Univ. of Science and Technology (Korea, Republic of)

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