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Journal of Biomedical Optics

Induction and imaging of photothrombotic stroke in conscious and freely moving rats
Author(s): Hongyang Lu; Yao Li; Lu Yuan; Hangdao Li; Xiaodan Lu; Shanbao Tong
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Paper Abstract

In experimental stroke research, anesthesia is common and serves as a major reason for translational failure. Real-time cerebral blood flow (CBF) monitoring during stroke onset can provide important information for the prediction of brain injury; however, this is difficult to achieve in clinical practice due to various technical problems. We created a photothrombotic focal ischemic stroke model utilizing our self-developed miniature headstage in conscious and freely moving rats. In this model, a high spatiotemporal resolution imager using laser speckle contrast imaging technology was integrated to acquire real-time two-dimensional CBF information during thrombosis. The feasibility, stability, and reliability of the system were tested in terms of CBF, behavior, and T2-weighted magnetic resonance imaging (MRI) findings. After completion of occlusion, the CBF in the targeted cortex of the stroke group was reduced to 16±9% of the baseline value. The mean infarct volume measured by MRI 24 h postmodeling was 77±11  mm3 and correlated well with CBF (R2=0.74). This rodent model of focal cerebral ischemia and real-time blood flow imaging opens the possibility of performing various fundamental and translational studies on stroke without the influence of anesthetics.

Paper Details

Date Published: 19 September 2014
PDF: 6 pages
J. Biomed. Opt. 19(9) 096013 doi: 10.1117/1.JBO.19.9.096013
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Hongyang Lu, Shanghai Jiao Tong Univ. (China)
Yao Li, Shanghai Jiao Tong Univ. (China)
Lu Yuan, Shanghai Jiao Tong Univ. (China)
Hangdao Li, Shanghai Jiao Tong Univ. (China)
Xiaodan Lu, Shanghai Jiao Tong Univ. (China)
Shanbao Tong, Shanghai Jiao Tong Univ. (China)

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