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Journal of Biomedical Optics

Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains
Author(s): Youting Chen; Jing Chen; Hong Chen; Zhipeng Hong; Xiaoqin Zhu; Shuangmu Zhuo; Yanling Chen; Jianxin Chen
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Paper Abstract

Hematoxylin and eosin (H&E) staining of tissue samples is the standard approach in histopathology for imaging and diagnosing cancer. Recent reports have shown that multiphoton microscopy (MPM) provides better sample interface with single-cell resolution, which enhances traditional H&E staining and offers a powerful diagnostic tool with potential applications in oncology. The purpose of this study was to further expand the versatility of MPM by establishing the optical parameters required for imaging unstained histological sections of pancreatic neoplasms, thereby providing an efficient and environmentally sustainable alternative to H&E staining while improving the accuracy of pancreatic cancer diagnoses. We found that the high-resolution MPM images clearly distinguish between the structure of normal pancreatic tissues compared with pancreatic neoplasms in unstained histological sections, and discernable differences in tissue architecture and cell morphology between normal versus tumorigenic cells led to enhanced optical diagnosis of cancerous tissue. Moreover, quantitative assessment of the cytomorphological features visualized from MPM images showed significant differences in the nuclear–cytoplasmic ratios of pancreatic neoplasms compared with normal pancreas, as well as further distinguished pancreatic malignant tumors from benign tumors. These results indicate that the MPM could potentially serve as an optical tool for the diagnosis of pancreatic neoplasms in unstained histological sections.

Paper Details

Date Published: 12 September 2014
PDF: 9 pages
J. Biomed. Opt. 19(9) 096008 doi: 10.1117/1.JBO.19.9.096008
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Youting Chen, Fujian Medical Univ. (China)
Jing Chen, Fujian Normal Univ. (China)
Hong Chen, Fujian Medical Univ. (China)
Zhipeng Hong, Fujian Medical Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)
Yanling Chen, Fujian Medical Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)


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