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Journal of Biomedical Optics

Spatial mapping of drug delivery to brain tissue using hyperspectral spatial frequency-domain imaging
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Paper Abstract

We present an application of spatial frequency-domain imaging (SFDI) to the wide-field imaging of drug delivery to brain tissue. Measurements were compared with values obtained by a previously validated variation of diffuse reflectance spectroscopy, the method of optical pharmacokinetics (OP). We demonstrate a cross-correlation between the two methods for absorption extraction and drug concentration determination in both experimental tissue phantoms and freshly extracted rodent brain tissue. These methods were first used to assess intra-arterial (IA) delivery of cationic liposomes to brain tissue in Sprague Dawley rats under transient cerebral hypoperfusion. Results were found to be in agreement with previously published experimental data and pharmacokinetic models of IA drug delivery. We then applied the same scheme to evaluate IA mitoxantrone delivery to glioma-bearing rats. Good correlation was seen between OP and SFDI determined concentrations taken from normal and tumor averaged sites. This study shows the feasibility of mapping drug/tracer distributions and encourages the use of SFDI for spatial imaging of tissues for drug/tracer-tagged carrier deposition and pharmacokinetic studies.

Paper Details

Date Published: 8 September 2014
PDF: 9 pages
J. Biomed. Opt. 19(9) 096003 doi: 10.1117/1.JBO.19.9.096003
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Rajinder P. Singh-Moon, Columbia Univ. Medical Ctr. (United States)
Darren M. Roblyer, Boston Univ. (United States)
Irving J. Bigio, Boston Univ. (United States)
Shailendra Joshi, Columbia Univ. (United States)


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