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Journal of Biomedical Optics • Open Access

Multiphoton microscopy in defining liver function
Author(s): Camilla A. Thorling; Darrell Crawford; Frank J. Burczynski; Xin Liu; Ian Liau; Michael S. Roberts

Paper Abstract

Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P)H changes with fluorescence lifetime imaging microscopy. The morphology of hepatocytes can be visualized without exogenously administered fluorescent dyes by utilizing their autofluorescence and second harmonic generation signal of collagen, which is useful in diagnosing liver disease. More specific imaging, such as studying drug transport in normal and diseased livers are achievable, but require exogenously administered fluorescent dyes. If these techniques can be translated into clinical use to assess liver function, it would greatly improve early diagnosis of organ viability, fibrosis, and cancer.

Paper Details

Date Published: 8 September 2014
PDF: 11 pages
J. Biomed. Opt. 19(9) 090901 doi: 10.1117/1.JBO.19.9.090901
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Camilla A. Thorling, Univ. of South Australia (Australia)
The Univ. of Queensland School of Medicine (Australia)
Darrell Crawford, The Univ. of Queensland School of Medicine (Australia)
Frank J. Burczynski, Univ. of Manitoba (Canada)
Xin Liu, The Univ. of Queensland School of Medicine (Australia)
Ian Liau, National Chiao Tung Univ. (Taiwan)
Michael S. Roberts, Univ. of South Australia (Australia)
The Univ. of Queensland School of Medicine (Australia)

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