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Journal of Biomedical Optics • Open Access

Fast and automatic imaging of immunoenzyme-stained neuronal circuits in the whole brain of <italic<Drosophila</italic<
Author(s): Qingping Tian; Jing Yuan; Yuxin Li; Tao Jiang; Hui Gong; Wei Zhou

Paper Abstract

Knowledge of neuronal wiring and morphogenesis in <italic<Drosophila</italic< is essential to understand brain function and dysfunction. The immunoenzyme method based on horseradish peroxidase/diaminobenzidine (HRP/DAB) provides high-contrast images to resolve details underlying neuronal architecture. However, the poor staining penetration and a lack of corresponding three-dimensional imaging methodology limit its application. Herein, we modified the HRP/DAB method to stain neuronal circuits in the whole brain of <italic<Drosophila</italic<. Furthermore, we found that imaging with the micro-optical sectioning tomography system provided a fast and automatic method that could dissect cell-specific neuroanatomical architecture at a submicron voxel resolution.

Paper Details

Date Published: 25 September 2014
PDF: 3 pages
J. Biomed. Opt. 19(9) 090506 doi: 10.1117/1.JBO.19.9.090506
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Qingping Tian, Huazhong Univ. of Science and Technology (China)
Jing Yuan, Huazhong Univ. of Science and Technology (China)
Yuxin Li, Huazhong Univ. of Science and Technology (China)
Tao Jiang, Huazhong Univ. of Science and Technology (China)
Hui Gong, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)


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