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Journal of Biomedical Optics • Open Access

Real-time absorption reduced surface fluorescence imaging
Author(s): Bin Yang; James W. Tunnell

Paper Abstract

We introduce a technique that limits absorption effects in fluorescence imaging and does not require extensive imaging processing, thus allowing for video rate imaging. The absorption minimization is achieved using spatial frequency domain imaging at a single high spatial frequency with standard three-phase demodulation. At a spatial frequency f=0.5  mm−1, we demonstrated in both in-vitro phantoms and ex-vivo tissue that the absorption can be significantly reduced. In the real-time implementation, we achieved a video rate of 19  frames/s. This technique has potential in cancer visualization and tumor margin detection.

Paper Details

Date Published: 24 September 2014
PDF: 3 pages
J. Biomed. Opt. 19(9) 090505 doi: 10.1117/1.JBO.19.9.090505
Published in: Journal of Biomedical Optics Volume 19, Issue 9
Show Author Affiliations
Bin Yang, The Univ. of Texas at Austin (United States)
James W. Tunnell, The Univ. of Texas at Austin (United States)

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