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Journal of Biomedical Optics • Open Access

Method measuring oxygen tension and transport within subcutaneous devices
Author(s): John Weidling; Sara Sameni; Jonathan R. Lakey; Elliot L. Botvinick

Paper Abstract

Cellular therapies hold promise to replace the implantation of whole organs in the treatment of disease. For most cell types, <italic<in vivo</italic< viability depends on oxygen delivery to avoid the toxic effects of hypoxia. A promising approach is the <italic<in situ</italic< vascularization of implantable devices which can mediate hypoxia and improve both the lifetime and utility of implanted cells and tissues. Although mathematical models and bulk measurements of oxygenation in surrounding tissue have been used to estimate oxygenation within devices, such estimates are insufficient in determining if supplied oxygen is sufficient for the entire thickness of the implanted cells and tissues. We have developed a technique in which oxygen-sensitive microparticles (OSMs) are incorporated into the volume of subcutaneously implantable devices. Oxygen partial pressure within these devices can be measured directly <italic<in vivo</italic< by an optical probe placed on the skin surface. As validation, OSMs have been incorporated into alginate beads, commonly used as immunoisolation devices to encapsulate pancreatic islet cells. Alginate beads were implanted into the subcutaneous space of Sprague–Dawley rats. Oxygen transport through beads was characterized from dynamic OSM signals in response to changes in inhaled oxygen. Changes in oxygen dynamics over days demonstrate the utility of our technology.

Paper Details

Date Published: 27 August 2014
PDF: 5 pages
J. Biomed. Opt. 19(8) 087006 doi: 10.1117/1.JBO.19.8.087006
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
John Weidling, Univ. of California, Irvine (United States)
Sara Sameni, Univ. of California, Irvine (United States)
Jonathan R. Lakey, Univ. of California, Irvine (United States)
Elliot L. Botvinick, Univ. of California, Irvine (United States)

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