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Journal of Biomedical Optics

<italic<In vivo</italic< Raman spectroscopy of human uterine cervix: exploring the utility of vagina as an internal control
Author(s): Rubina S. Shaikh; Tapas Kumar Dora; Supriya Chopra; Amita Maheshwari; Deodhar K. Kedar; Rekhi Bharat; C. Murali Krishna
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Paper Abstract

<italic<In vivo</italic< Raman spectroscopy is being projected as a new, noninvasive method for cervical cancer diagnosis. In most of the reported studies, normal areas in the cancerous cervix were used as control. However, in the Indian subcontinent, the majority of cervical cancers are detected at advanced stages, leaving no normal sites for acquiring control spectra. Moreover, vagina and ectocervix are reported to have similar biochemical composition. Thus, in the present study, we have evaluated the feasibility of classifying normal and cancerous conditions in the Indian population and we have also explored the utility of the vagina as an internal control. A total of 228 normal and 181 tumor <italic<in vivo</italic< Raman spectra were acquired from 93 subjects under clinical supervision. The spectral features in normal conditions suggest the presence of collagen, while DNA and noncollagenous proteins were abundant in tumors. Principal-component linear discriminant analysis (PC-LDA) yielded 97% classification efficiency between normal and tumor groups. An analysis of a normal cervix and vaginal controls of cancerous and noncancerous subjects suggests similar spectral features between these groups. PC-LDA of tumor, normal cervix, and vaginal controls further support the utility of the vagina as an internal control. Overall, findings of the study corroborate with earlier studies and facilitate objective, noninvasive, and rapid Raman spectroscopic-based screening/diagnosis of cervical cancers.

Paper Details

Date Published: 7 August 2014
PDF: 8 pages
J. Biomed. Opt. 19(8) 087001 doi: 10.1117/1.JBO.19.8.087001
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Rubina S. Shaikh, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Tapas Kumar Dora, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Supriya Chopra, Advanced Ctr. for Treatment, Research & Education in Cancer (India)
Amita Maheshwari, Tata Memorial Hospital (India)
Deodhar K. Kedar, Tata Memorial Hospital (India)
Rekhi Bharat, Tata Memorial Hospital (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research & Education in Cancer (India)


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