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Journal of Biomedical Optics • Open Access

Quantitative short-wave infrared multispectral imaging of <italic<in vivo</italic< tissue optical properties
Author(s): Robert H. Wilson; Kyle P. Nadeau; Frank B. Jaworski; Rebecca Rowland; John Quan M. Nguyen; Christian Crouzet; Rolf B. Saager; Bernard Choi; Bruce J. Tromberg; Anthony J. Durkin

Paper Abstract

Extending the wavelength range of spatial frequency domain imaging (SFDI) into the short-wave infrared (SWIR) has the potential to provide enhanced sensitivity to chromophores such as water and lipids that have prominent absorption features in the SWIR region. Here, we present, for the first time, a method combining SFDI with unstructured (zero spatial frequency) illumination to extract tissue absorption and scattering properties over a wavelength range (850 to 1800 nm) largely unexplored by previous tissue optics techniques. To obtain images over this wavelength range, we employ a SWIR camera in conjunction with an SFDI system. We use SFDI to obtain <italic<in vivo</italic< tissue reduced scattering coefficients at the wavelengths from 850 to 1050 nm, and then use unstructured wide-field illumination and an extrapolated power-law fit to this scattering spectrum to extract the absorption spectrum from 850 to 1800 nm. Our proof-of-principle experiment in a rat burn model illustrates that the combination of multispectral SWIR imaging, SFDI, and unstructured illumination can characterize <italic<in vivo</italic< changes in skin optical properties over a greatly expanded wavelength range. In the rat burn experiment, these changes (relative to normal, unburned skin) included increased absorption and increased scattering amplitude and slope, consistent with changes that we previously reported in the near-infrared using SFDI.

Paper Details

Date Published: 13 August 2014
PDF: 4 pages
J. Biomed. Opt. 19(8) 086011 doi: 10.1117/1.JBO.19.8.086011
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Robert H. Wilson, Beckman Laser Institute and Medical Clinic (United States)
Kyle P. Nadeau, Beckman Laser Institute and Medical Clinic (United States)
Frank B. Jaworski, Raytheon Vision Systems (United States)
Rebecca Rowland, Beckman Laser Institute and Medical Clinic (United States)
John Quan M. Nguyen, Beckman Laser Institute and Medical Clinic (United States)
Christian Crouzet, Beckman Laser Institute and Medical Clinic (United States)
Rolf B. Saager, Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)


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