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Journal of Biomedical Optics

Multispectral measurement of contrast in tissue-mimicking phantoms in near-infrared spectral range of 650 to 1600 nm
Author(s): Daniel Salo; Hairong Zhang; David M. Kim; Mikhail Y. Berezin
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Paper Abstract

In order to identify the optimal imaging conditions for the highest spatial contrast in biological tissue, we explored the properties of a tissue-mimicking phantom as a function of the wavelengths in a broad range of near-infrared spectra (650 to 1600 nm). Our customized multispectral hardware, which featured a scanning transmission microscope and imaging spectrographs equipped with silicon and InGaAs charge-coupled diode array detectors, allowed for direct comparison of the Michelson contrast obtained from a phantom composed of a honeycomb grid, Intralipid, and India ink. The measured contrast depended on the size of the grid, luminance, and the wavelength of measurements. We demonstrated that at low thickness of the phantom, a reasonable contrast of the objects can be achieved at any wavelength between 700 and 1400 nm and between 1500 and 1600 nm. At larger thicknesses, such contrast can be achieved mostly between 1200 and 1350 nm. These results suggest that distinguishing biological features in deep tissue and developing contrast agents for <italic<in vivo</italic< may benefit from imaging in this spectral range.

Paper Details

Date Published: 7 August 2014
PDF: 7 pages
J. Biomed. Opt. 19(8) 086008 doi: 10.1117/1.JBO.19.8.086008
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Daniel Salo, Washington Univ. School of Medicine in St. Louis (United States)
Hairong Zhang, Washington Univ. School of Medicine in St. Louis (United States)
David M. Kim, Washington Univ. School of Medicine in St. Louis (United States)
Mikhail Y. Berezin, Washington Univ. School of Medicine in St. Louis (United States)

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