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Journal of Biomedical Optics

Label-free real-time imaging of myelination in the <italic<Xenopus laevis</italic< tadpole by <italic<in vivo</italic< stimulated Raman scattering microscopy
Author(s): Chun-Rui Hu; Delong Zhang; Mikhail N. Slipchenko; Ji-Xin Cheng; Bing Hu
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Paper Abstract

The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time <italic<in vivo</italic< imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the <italic<Xenopus laevis</italic< tadpole.

Paper Details

Date Published: 7 August 2014
PDF: 8 pages
J. Biomed. Opt. 19(8) 086005 doi: 10.1117/1.JBO.19.8.086005
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Chun-Rui Hu, Univ. of Science and Technology of China (China)
Delong Zhang, Purdue Univ. (United States)
Mikhail N. Slipchenko, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)
Bing Hu, Univ. of Science and Technology of China (China)

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