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Journal of Biomedical Optics

Additivity of light-scattering patterns of aggregated biological particles
Author(s): Alexander E. Moskalensky; Dmitry I. Strokotov; Andrei V. Chernyshev; Valeri P. Maltsev; Maxim A. Yurkin
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Paper Abstract

The paper is focused on light scattering by aggregates of optically soft particles with a size larger than the wavelength, in particular, blood platelets. We conducted a systematic simulation of light scattering by dimers and larger aggregates of blood platelets, each modeled as oblate spheroids, using the discrete dipole approximation. Two-dimensional (2-D) light scattering patterns (LSPs) and internal fields showed that the multiple scattering between constituent particles can be neglected. Additionally, we derived conditions of the scattering angle and orientation of the dimer, under which the averaging of the 2-D LSPs over the azimuthal scattering angle washes out interference in the far field, resulting in averaged LSPs of the aggregate being equal to the sum of that for its constituents. We verified theoretical conclusions using the averaged LSPs of blood platelets measured with the scanning flow cytometer (SFC). Moreover, we obtained similar results for a model system of aggregates of polystyrene beads, studied both experimentally and theoretically. Finally, we discussed the potential of discriminating platelet aggregates from monomers using the SFC.

Paper Details

Date Published: 7 August 2014
PDF: 11 pages
J. Biomed. Opt. 19(8) 085004 doi: 10.1117/1.JBO.19.8.085004
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Alexander E. Moskalensky, Institute of Chemical Kinetics and Combustion (Russia)
Novosibirsk State Univ. (Russian Federation)
Dmitry I. Strokotov, Institute of Chemical Kinetics and Combustion (Russian Federation)
Novosibirsk State Univ. (Russian Federation)
Andrei V. Chernyshev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Novosibirsk State Univ. (Russian Federation)
Valeri P. Maltsev, Institute of Chemical Kinetics and Combustion (Russian Federation)
Novosibirsk State Univ. (Russian Federation)
Novosibirsk State Medical Univ. (Russian Federation)
Maxim A. Yurkin, Institute of Chemical Kinetics and Combustion (Russian Federation)
Novosibirsk State Univ. (Russian Federation)


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