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Journal of Biomedical Optics

Autofluorescence imaging device for real-time detection and tracking of pathogenic bacteria in a mouse skin wound model: preclinical feasibility studies
Author(s): Yichao C. Wu; Iris Kulbatski; Philip J. Medeiros; Azusa Maeda; Jiachuan Bu; Lizhen Xu; Yonghong Chen; Ralph S. DaCosta
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Paper Abstract

Bacterial infection significantly impedes wound healing. Clinical diagnosis of wound infections is subjective and suboptimal, in part because bacteria are invisible to the naked eye during clinical examination. Moreover, bacterial infection can be present in asymptomatic patients, leading to missed opportunities for diagnosis and treatment. We developed a prototype handheld autofluorescence (AF) imaging device (Portable Real-time Optical Detection, Identification and Guidance for Intervention—PRODIGI) to noninvasively visualize and measure bacterial load in wounds in real time. We conducted preclinical pilot studies in an established nude mouse skin wound model inoculated with bioluminescent <italic<Staphylococcus aureus</italic< bacteria. We tested the feasibility of longitudinal AF imaging for <italic<in vivo</italic< visualization of bacterial load in skin wounds, validated by bioluminescence imaging. We showed that bacteria (<italic<S. aureus</italic<), occult to standard examination, can be visualized in wounds using PRODIGI. We also detected quantitative changes in wound bacterial load over time based on the antibiotic treatment and the correlation of bacterial AF intensity with bacterial load. AF imaging of wounds offers a safe, noninvasive method for visualizing the presence, location, and extent of bacteria as well as measuring relative changes in bacterial load in wounds in real time.

Paper Details

Date Published: 4 August 2014
PDF: 9 pages
J. Biomed. Opt. 19(8) 085002 doi: 10.1117/1.JBO.19.8.085002
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Yichao C. Wu, Princess Margaret Hospital (Canada)
University Health Network (Canada)
Iris Kulbatski, Princess Margaret Hospital (Canada)
University Health Network (Canada)
Philip J. Medeiros, Princess Margaret Hospital (Canada)
University Health Network (Canada)
Azusa Maeda, Princess Margaret Hospital (Canada)
Univ. of Toronto (Canada)
University Health Network (Canada)
Jiachuan Bu, Princess Margaret Hospital (Canada)
University Health Network (Canada)
Lizhen Xu, Univ. of Toronto (Canada)
University Health Network (Canada)
Yonghong Chen, Ontario Cancer Institute (Canada)
University Health Network (Canada)
Princess Margaret Hospital (Canada)
Ralph S. DaCosta, Univ. Health Network (Canada)
Univ. of Toronto (Canada)


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