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Journal of Biomedical Optics • Open Access

Review of recent developments in stimulated emission depletion microscopy: applications on cell imaging
Author(s): Bhanu Neupane; Frances S. Ligler; Gufeng Wang

Paper Abstract

Stimulated emission depletion (STED) microscopy is one type of far-field optical technique demonstrated to provide subdiffraction resolution. STED microscopy utilizes a donut-shaped depletion beam to limit the probe volume to be much smaller than a diffraction-limited spot. Resolutions as small as a few tens of nanometers laterally are reported for cell analysis. The different versions of STED microscopes are described and contrasted in terms of their applicability for biological imaging. Finally, we suggest likely avenues for improving the performance and increasing the utility of STED microscopy.

Paper Details

Date Published: 14 August 2014
PDF: 9 pages
J. Biomed. Opt. 19(8) 080901 doi: 10.1117/1.JBO.19.8.080901
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Bhanu Neupane, The Univ. of North Carolina at Chapel Hill (United States)
North Carolina State Univ. (United States)
Frances S. Ligler, The Univ. of North Carolina at Chapel Hill and North Carolina State University (United States)
Gufeng Wang, North Carolina State Univ. (United States)


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