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Journal of Biomedical Optics

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts
Author(s): Hussain Fatakdawala; Leigh G. Griffiths; Sterling Humphrey; Laura Marcu
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Paper Abstract

Quantitative and qualitative evaluations of structure and composition are important in monitoring development of engineered vascular tissue both <italic<in vitro</italic< and <italic<in vivo</italic<. Destructive techniques are an obstacle for performing time-lapse analyses from a single sample or animal. This study demonstrates the ability of time-resolved fluorescence spectroscopy (TRFS) and ultrasound backscatter microscopy (UBM), as nondestructive and synergistic techniques, for compositional and morphological analyses of tissue grafts, respectively. UBM images and integrated backscatter coefficients demonstrate the ability to visualize and quantify postimplantation changes in vascular graft biomaterials such as loss of the external elastic lamina and intimal/medial thickening over the grafted region as well as graft integration with the surrounding tissue. TRFS results show significant changes in spectra, average lifetime, and fluorescence decay parameters owing to changes in collagen, elastin, and cellular content between normal and grafted tissue regions. These results lay the foundation for the application of a catheter-based technique for <italic<in vivo</italic< evaluation of vascular grafts using TRFS and UBM.

Paper Details

Date Published: 22 August 2014
PDF: 3 pages
J. Biomed. Opt. 19(8) 080503 doi: 10.1117/1.JBO.19.8.080503
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Hussain Fatakdawala, Univ. of California, Davis (United States)
Leigh G. Griffiths, Univ. of California, Davis (United States)
Sterling Humphrey, Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)


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