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Journal of Biomedical Optics • Open Access

Optimization of sample cooling temperature for redox cryo-imaging
Author(s): Anle Wang; Jing Yuan; Weihua Luo; Mengmeng Liu; Qingming Luo

Paper Abstract

Cryo-imaging techniques have been widely used to measure the metabolic state of tissues by capturing reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) autofluorescence. However, NADH and FAD fluorescence is sensitive to changes in temperature, which may result in unreliable redox ratio calculations. Here, the relationship between the measured redox ratio and sample surface temperature was analyzed using a standard phantom solution and biological tissues. The results indicated that a temperature <−100°C was a suitable cryo-imaging temperature window in which redox ratio measuring was immune to temperature fluctuations. These results may serve as a reference for designing and optimizing redox cryo-imaging experiments for quantitatively mapping the metabolic state of biological samples.

Paper Details

Date Published: 22 August 2014
PDF: 3 pages
J. Biomed. Opt. 19(8) 080502 doi: 10.1117/1.JBO.19.8.080502
Published in: Journal of Biomedical Optics Volume 19, Issue 8
Show Author Affiliations
Anle Wang, Britton Chance Ctr. for Biomedical Photonics (China)
Wuhan National Lab. for Optoelectronics (China)
Jing Yuan, Huazhong Univ. of Science and Technology (China)
Weihua Luo, Huazhong Univ. of Science and Technology (China)
Mengmeng Liu, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)


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