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Journal of Biomedical Optics

In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate
Author(s): Veronika Huntosova; Sandrine Gay; Patrycja M. Nowak-Sliwinska; Senthil K. Rajendran; Matthieu Zellweger; Hubert van den Bergh; Georges Wagnières
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Paper Abstract

Measuring tissue oxygenation in vivo is of interest in fundamental biological as well as medical applications. One minimally invasive approach to assess the oxygen partial pressure in tissue (pO2) is to measure the oxygen-dependent luminescence lifetime of molecular probes. The relation between tissue pO2 and the probes’ luminescence lifetime is governed by the Stern-Volmer equation. Unfortunately, virtually all oxygen-sensitive probes based on this principle induce some degree of phototoxicity. For that reason, we studied the oxygen sensitivity and phototoxicity of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate [Ru(Phen)] using a dedicated optical fiber–based, time-resolved spectrometer in the chicken embryo chorioallantoic membrane. We demonstrated that, after intravenous injection, Ru(Phen)’s luminescence lifetime presents an easily detectable pO2 dependence at a low drug dose (1  mg/kg) and low fluence (120  mJ/cm2 at 470 nm). The phototoxic threshold was found to be at 10  J/cm2 with the same wavelength and drug dose, i.e., about two orders of magnitude larger than the fluence necessary to perform a pO2 measurement. Finally, an illustrative application of this pO2 measurement approach in a hypoxic tumor environment is presented.

Paper Details

Date Published: 18 July 2014
PDF: 12 pages
J. Biomed. Opt. 19(7) 077004 doi: 10.1117/1.JBO.19.7.077004
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Veronika Huntosova, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Sandrine Gay, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Patrycja M. Nowak-Sliwinska, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Ctr. Hospitalier Univ. Vaudois (Switzerland)
Senthil K. Rajendran, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Matthieu Zellweger, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Hubert van den Bergh, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Georges Wagnières, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

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