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Journal of Biomedical Optics

Accurate extraction of optical properties and top layer thickness of two-layered mucosal tissue phantoms from spatially resolved reflectance spectra
Author(s): Kung-Bin Sung; Kuang-Wei Shih; Fang-Wei Hsu; Hong-Po Hsieh; Min-Jie Chuang; Yi-Hsien Hsiao; Yu-Hui Su; Gen-Hao Tien
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Paper Abstract

We are reporting on an experimental investigation of a movable diffuse reflectance spectroscopy system to extract diagnostically relevant optical properties of two-layered tissue phantoms simulating mucosae that are covered with stratified squamous epithelium. The reflectance spectra were measured at multiple source-detector separations using two imaging fiber bundles in contact with the phantoms, one with its optical axis perpendicular to the sample surface (perpendicular probe) and the other with its distal end beveled and optical axis tilted at 45 deg (oblique probe). Polystyrene microspheres and purified human hemoglobin were used to make tissue phantoms whose scattering and absorption properties could be well controlled and theoretically predicted. Monte Carlo simulations were used to predict the reflectance spectra for system calibration and an iterative curve fitting that simultaneously extracted the top layer reduced scattering coefficient, thickness, bottom layer reduced scattering coefficient, and hemoglobin concentration of the phantoms. The errors of the recovered parameters ranged from 7% to 20%. The oblique probe showed higher accuracy in the extracted top layer reduced scattering coefficient and thickness than the perpendicular probe. The developed system and data analysis methods provide a feasible tool to quantify the optical properties <italic<in vivo</italic<.

Paper Details

Date Published: 15 July 2014
PDF: 9 pages
J. Biomed. Opt. 19(7) 077002 doi: 10.1117/1.JBO.19.7.077002
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Kung-Bin Sung, National Taiwan Univ. (Taiwan)
Kuang-Wei Shih, National Taiwan Univ. (Taiwan)
Fang-Wei Hsu, National Taiwan Univ. (Taiwan)
Hong-Po Hsieh, National Taiwan Univ. (Taiwan)
Min-Jie Chuang, National Taiwan Univ. (Taiwan)
Yi-Hsien Hsiao, National Taiwan Univ. (Taiwan)
Yu-Hui Su, National Taiwan Univ. (Taiwan)
Gen-Hao Tien, National Taiwan Univ. (Taiwan)


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