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Journal of Biomedical Optics

Assessment of microcirculation dynamics during cutaneous wound healing phases <italic<in vivo</italic< using optical microangiography
Author(s): Siavash Yousefi; Jia J. Qin; Suzan Dziennis; Ruikang K. Wang
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Paper Abstract

Cutaneous wound healing consists of multiple overlapping phases starting with blood coagulation following incision of blood vessels. We utilized label-free optical coherence tomography and optical microangiography (OMAG) to noninvasively monitor healing process and dynamics of microcirculation system in a mouse ear pinna wound model. Mouse ear pinna is composed of two layers of skin separated by a layer of cartilage and because its total thickness is around 500 <italic<μ</italic<m, it can be utilized as an ideal model for optical imaging techniques. These skin layers are identical to human skin structure except for sweat ducts and glands. Microcirculatory system responds to the wound injury by recruiting collateral vessels to supply blood flow to hypoxic region. During the inflammatory phase, lymphatic vessels play an important role in the immune response of the tissue and clearing waste from interstitial fluid. In the final phase of wound healing, tissue maturation, and remodeling, the wound area is fully closed while blood vessels mature to support the tissue cells. We show that using OMAG technology allows noninvasive and label-free monitoring and imaging each phase of wound healing that can be used to replace invasive tissue sample histology and immunochemistry technologies.

Paper Details

Date Published: 18 July 2014
PDF: 11 pages
J. Biomed. Opt. 19(7) 076015 doi: 10.1117/1.JBO.19.7.076015
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Siavash Yousefi, Univ. of Washington (United States)
Jia J. Qin, Univ. of Washington (United States)
Suzan Dziennis, Univ. of Washington (United States)
Ruikang K. Wang, Univ. of Washington (United States)


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