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Journal of Biomedical Optics

Delayed near-infrared analysis permits visualization of rodent retinal pigment epithelium layer <italic<in vivo</italic<
Author(s): Natalie Pankova; Xu Zhao; Huiyuan Liang; David Sung Hyeon Baek; Hai Wang; Shelley Boyd
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Paper Abstract

Patches of atrophy of the retinal pigment epithelium (RPE) have not been described in rodent models of retinal degeneration, as they have the clinical setting using fundus autofluorescence. We hypothesize that prelabeling the RPE would increase contrast and allow for improved visualization of RPE loss <italic<in vivo</italic<. Here, we demonstrate a new technique termed “delayed near-infrared analysis (DNIRA)” that permits ready detection of rat RPE, using optical imaging in the near-infrared (IR) spectrum with aid of indocyanine green (ICG) dye. Using DNIRA, we demonstrate a fluorescent RPE signal that is detected using confocal scanning laser ophthalmoscopy up to 28 days following ICG injection. This signal is apparent only after ICG injection, is dose dependent, requires the presence of the ICG filters (795/810 nm excitation/emission), does not appear in the IR reflectance channel, and is eliminated in the presence of sodium iodate, a toxin that causes RPE loss. Rat RPE explants confirm internalization of ICG dye. Together with normal retinal electrophysiology, these findings demonstrate that DNIRA is a new and safe noninvasive optical imaging technique for <italic<in vivo</italic< visualization of the RPE in models of retinal disease.

Paper Details

Date Published: 8 July 2014
PDF: 8 pages
J. Biomed. Opt. 19(7) 076007 doi: 10.1117/1.JBO.19.7.076007
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Natalie Pankova, Univ. of Toronto (Canada)
St. Michael's Hospital (Canada)
Xu Zhao, St. Michael's Hospital (Canada)
Huiyuan Liang, St. Michael's Hospital (Canada)
David Sung Hyeon Baek, Univ. of Toronto (Canada)
St. Michael’s Hospital (Canada)
Hai Wang, St. Michael's Hospital (Canada)
Shelley Boyd, Univ. of Toronto (Canada)
St. Michael's Hospital (Canada)

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