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Journal of Biomedical Optics • Open Access

Characterization, comparison, and choice of a commercial double-clad fiber for nonlinear endomicroscopy

Paper Abstract

Several endomicroscope prototypes for nonlinear optical imaging were developed in the last decade for <italic<in situ</italic< analysis of tissue with cellular resolution by using short infrared light pulses. Fourier-transform-limited pulses at the tissue site are necessary for optimal excitation of faint endogenous signals. However, obtaining these transform-limited short pulses remains a challenge, and previously proposed devices did not achieve an optimal pulse delivery. We present a study of fibered endomicroscope architecture with an efficient femtosecond pulse delivery and a high excitation level at the output of commercially available double-clad fibers (DCFs). The endomicroscope incorporates a module based on a grism line to compensate for linear and nonlinear effects inside the system. Simulations and experimental results are presented and compared to the literature. Experimentally, we obtained short pulses down to 24 fs at the fiber output, what represents to the best of our knowledge the shortest pulse duration ever obtained at the output of a nonlinear endoscopic system without postcompression. The choice of the optimal DCF among four possible commercial components is discussed and evaluated in regard to multiphoton excitation and fluorescence emission.

Paper Details

Date Published: 7 July 2014
PDF: 8 pages
J. Biomed. Opt. 19(7) 076005 doi: 10.1117/1.JBO.19.7.076005
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Claire Lefort, Paris-Sud Univ. 11 (France)
Univ. de Limoges (France)
Hussein Hamzeh, Paris-Sud Univ. 11 (France)
Frederic Louradour, Univ. de Limoges (France)
XLIM Lab. (France)
Frédéric Pain, Paris-Sud Univ. 11 (France)
D. Abi Haidar, Univ. Paris-Sud 11 (France)
Univ. Paris 7-Denis Diderot (France)

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