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Journal of Biomedical Optics • Open Access

Three-dimensional correction of conduction velocity in the embryonic heart using integrated optical mapping and optical coherence tomography
Author(s): Pei Ma; Yves T. Wang; Shi Gu; Michiko Watanabe; Michael W. Jenkins; Andrew M. Rollins

Paper Abstract

Optical mapping (OM) of cardiac electrical activity conventionally collects information from a three-dimensional (3-D) surface as a two-dimensional (2-D) projection map. When applied to measurements of the embryonic heart, this method ignores the substantial and complex curvature of the heart surface, resulting in significant errors when calculating conduction velocity, an important electrophysiological parameter. Optical coherence tomography (OCT) is capable of imaging the 3-D structure of the embryonic heart and accurately characterizing the surface topology. We demonstrate an integrated OCT/OM imaging system capable of simultaneous conduction mapping and 3-D structural imaging. From these multimodal data, we obtained 3-D activation maps and corrected conduction velocity maps of early embryonic quail hearts. 3-D correction eliminates underestimation bias in 2-D conduction velocity measurements, therefore enabling more accurate measurements with less experimental variability. The integrated system will also open the door to correlate the structure and electrophysiology, thereby improving our understanding of heart development.

Paper Details

Date Published: 4 July 2014
PDF: 9 pages
J. Biomed. Opt. 19(7) 076004 doi: 10.1117/1.JBO.19.7.076004
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Pei Ma, Case Western Reserve Univ. (United States)
Yves T. Wang, Case Western Reserve Univ. (United States)
Shi Gu, Case Western Reserve Univ. (United States)
Michiko Watanabe, Case Western Reserve Univ. (United States)
Michael W. Jenkins, Case Western Reserve Univ. (United States)
Andrew M. Rollins, Case Western Reserve Univ. (United States)

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