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Journal of Biomedical Optics • Open Access

Biological imaging with coherent Raman scattering microscopy: a tutorial
Author(s): Alba Alfonso-Garcia; Richa Mittal; Eun Seong Lee; Eric O. Potma

Paper Abstract

Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research.

Paper Details

Date Published: 10 March 2014
PDF: 14 pages
J. Biomed. Opt. 19(7) 071407 doi: 10.1117/1.JBO.19.7.071407
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Alba Alfonso-Garcia, Beckman Laser Institute and Medical Clinic (United States)
Richa Mittal, Beckman Laser Institute and Medical Clinic (United States)
Eun Seong Lee, Korea Research Institute of Standards and Science (Korea, Republic of)
Eric O. Potma, Beckman Laser Institute and Medical Clinic (United States)


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