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Journal of Biomedical Optics • Open Access

Phototoxic effects of lysosome-associated genetically encoded photosensitizer KillerRed
Author(s): Ekaterina O. Serebrovskaya; Alina P. Ryumina; Maria E. Boulina; Marina V. Shirmanova; Elena V. Zagaynova; Ekaterina A. Bogdanova; Sergey A. Lukyanov; Konstantin A. Lukyanov

Paper Abstract

KillerRed is a unique phototoxic red fluorescent protein that can be used to induce local oxidative stress by green-orange light illumination. Here we studied phototoxicity of KillerRed targeted to cytoplasmic surface of lysosomes via fusion with Rab7, a small GTPase that is known to be attached to membranes of late endosomes and lysosomes. It was found that lysosome-associated KillerRed ensures efficient light-induced cell death similar to previously reported mitochondria- and plasma membrane–localized KillerRed. Inhibitory analysis demonstrated that lysosomal cathepsins play an important role in the manifestation of KillerRed-Rab7 phototoxicity. Time-lapse monitoring of cell morphology, membrane integrity, and nuclei shape allowed us to conclude that KillerRed-Rab7-mediated cell death occurs via necrosis at high light intensity or via apoptosis at lower light intensity. Potentially, KillerRed-Rab7 can be used as an optogenetic tool to direct target cell populations to either apoptosis or necrosis.

Paper Details

Date Published: 23 December 2013
PDF: 5 pages
J. Biomed. Opt. 19(7) 071403 doi: 10.1117/1.JBO.19.7.071403
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Ekaterina O. Serebrovskaya, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Alina P. Ryumina, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Maria E. Boulina, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Marina V. Shirmanova, Nizhni Novgorod State Medical Academy (Russian Federation)
Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
Ekaterina A. Bogdanova, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Sergey A. Lukyanov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)
Konstantin A. Lukyanov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Russian Federation)


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