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Journal of Biomedical Optics

Effects of tissue fixation on coherent anti-Stokes Raman scattering images of brain
Author(s): Roberta Galli; Ortrud Uckermann; Edmund Koch; Gabriele Schackert; Matthias Kirsch; Gerald Steiner
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Paper Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging multiphoton technique for the label-free histopathology of the central nervous system, by imaging the lipid content within the tissue. In order to apply the technique on standard histology sections, it is important to know the effects of tissue fixation on the CARS image. Here, we report the effects of two common fixation methods, namely with formalin and methanol–acetone, on mouse brain and human glioblastoma tissue. The variations induced by fixation on the CARS contrast and intensity were compared and interpreted using Raman microspectroscopy. The results show that, whenever unfixed cryosections cannot be used, fixation with formalin constitutes an alternative which does not deteriorate substantially the contrast generated by the different brain structures in the CARS image. Fixation with methanol–acetone strongly modifies the tissue lipid content and is therefore incompatible with the CARS imaging.

Paper Details

Date Published: 23 December 2013
PDF: 8 pages
J. Biomed. Opt. 19(7) 071402 doi: 10.1117/1.JBO.19.7.071402
Published in: Journal of Biomedical Optics Volume 19, Issue 7
Show Author Affiliations
Roberta Galli, Technische Univ. Dresden (Germany)
Ortrud Uckermann, Technische Univ. Dresden (Germany)
Edmund Koch, Technische Univ. Dresden (Germany)
Gabriele Schackert, Technische Univ. Dresden (Germany)
Matthias Kirsch, Technische Univ. Dresden (Germany)
Gerald Steiner, Technische Univ. Dresden (Germany)

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